Offering access to advanced microscopy systems

The LCIF is operated by the Neuroscience Research Program out of the Kleysen Institute for Advanced Medicine since it’s establishment in 2019.  The LCIF maintains advanced microscopy systems for imaging both live and fixed cells.  It is available for use across campuses in Manitoba.

The microscope systems include:

Zeiss LSM 880

Capabilities

  • Super Resolution Imaging with AiryScan (1.7X improvement over diffraction limited imaging)
  • Multichannel Imaging
  • Spectral Imaging and Unmixing
  • Live Cell Imaging
  • FRET, FRAP, FLIP Photo-Activation/-Conversion
  • Z-Stack, Tile Scanning, Timelapse

Microscope

  • Zeiss Axio Observer Z1
  • Motorized XY scanning stage
  • Motorized focus control (Z) with Definite Focus
  • Stage Top Chamber with Environmental Support (temperature, humidity and CO2)

Objectives

  • 5x, 0.13 NA
  • 20x, 0.8 NA
  • 40x, 1.2 NA, Water Corr
  • 63x, 1.3 NA

Illumination

  • Transmitted Light Illuminator (Halogen lamp)
  • Epi Fluorescence Illuminator (HXP 120V; widefield visual observation)
  • Laser: 405nm, multiline argon (458nm, 488nm, 514nm), 561nm, 633nm

Detectors

  • 2 Conventional PMTs
  • 1 High sensitivity GaAsP detector
  • 1 AiryScan Super Resolution Detector
Zeiss PALM

Capabilities

  • Microbeam laser unit (pulsed 355nm) allowing laser cutting and non-contact capture of both fixed and live samples
  • Multichannel imaging
  • Live cell imaging
  • Timelapse, time series, timed capture

Microscope

  • Zeiss Axio Observer Z1
  • Motorized XY scanning stage and focus control (Z)
  • Stage top chamber with environmental support (temperature, humidity and CO2)

Objectives

  • Fluar 5x, 0.25 NA
  • Fluar 10x, 0.25 NA
  • LD Plan-Neofluar 20x, 0.4 NA Corr
  • LD Plan-Neofluar 40x, 0.6 NA Corr

Illumination

  • Transmitted light illuminator (Halogen lamp)
  • Epi fluorescence illuminator (HXP 120V)
  • Filter based excitation/emission for DAPI, GFP, mRFP, Cy5

Cameras

  • AxioCam ICc 5 - color camera
  • AxioCam MRm - monochrome camera
2 Photon - in vitro

Capabilities

  • Two-photon excitation with dual channel detection allows for deep tissue penetration and imaging of GFP together with mCherry/TdTomato
  • Multiarea (XYZ), time-lapse imaging with environment control (solution perfusion, O2/CO2, temperature) for live imaging in tissue sections.
  • Photostimulation, uncaging

Microscope

  • Olympus BX61WI upright microscope with motorized focus control (Z drive)
  • Fully motorized stage for precise repeatable movement (XY) of the specimen with respect to the fixed microscope
  • Stage top chamber with environmental support (temperature, humidity and C2)

Objectives

  • 10x, 0.3 NA water immersion
  • 40x, 0.8 NA water immersion

Illumination

  • Transmitted light illuminator (Halogen lamp)
  • Ti:S laser tuneable between 690 to 1040 nm

Detectors

  • 2 Photomultiplier detector pairs (non-descanned, above stage and substage): for high sensitivity capture of emitted fluorescence (both reflected and transmitted). Equipped with emission filters for two channel acquisition of GFP and mCherry/TdTomato
  • Transmitted light detector with Dodt gradient contrast
2 Photon - intravital

Capabilities

  • Two-photon excitation with dual channel detection allows deep tissue penetration and fluorescence imaging
  • The system is adaptable, with optional stage and specimen chamber available for imaging from tissue sections, but is primarily designed for imaging from live rodents, for example via a cranial window (thinned skull or open-skull glass window) at depths of up to 1 mm from the brain surface
  • Capable of live imaging at video frame paired with simultaneous photostimulation

Microscope

  • Olympus BX61WI upright microscope with motorized focus control (Z drive)
  • Fully motorized stage for precise repeatable movement (XY) of the specimen with respect to the fixed microscope is available
  • Stage top chamber with environmental support (temperature, humidity and O2/CO2) is available for use

Objectives

  • 10x, 0.3 NA water immersion
  • 20x, 1.0 NA water immersion
  • 40x, 0.8 NA water immersion

Illumination

  • Transmitted light illuminator (Halogen lamp)
  • Fast resonance scanner with inputs for two Ti:S lasers tuneable between 690 to 1040 nm, one of which is dedicated for photostimulation

Detectors

  • Two high sensitivity GaAsP detectors, configured to capture emitted fluorescence from up to three distinct fluorophores (e.g. blue, green or red fluorescent emitters) in real-time
  • Transmitted light detector with Dodt gradient contrast

Fees

Option 1: Frequent users

$50/hr for the first 100 hrs and $25/hr thereafter.  Find more details in the LCIF Policy Agreement.

Option 2: Power users

Pre-paid rate of $8000 for the fiscal year.  Includes unlimited access to microscope systems.  Find more details in the LCIF Policy Agreement.


Ask the LCIF a question

     


    Get access now

     

    1

    Read & complete the LCIF Policy Agreement

    Complete the LCIF Policy Agreement Be prepared to provide a FOP.

     

    Connect for training

    For Confocal systemDr. Michael F. Jackson

    For 2-photon systemsDr. Ping Lu

    For Palm Laser Capture Microdissection system: Dr. Tiina Kauppinen

    Include your signed agreement in your email and make arrangements for training.

    3

    Get access to the online calendar

    When training is complete and considered satisfactory, you will given access to the online LCIF Calendar.