Capabilities

• Super Resolution Imaging with AiryScan (1.7X improvement over diffraction limited imaging)
• Multichannel Imaging
• Spectral Imaging and Unmixing
• Live Cell Imaging
• FRET, FRAP, FLIP Photo-Activation/-Conversion
• Z-Stack, Tile Scanning, Timelapse

Microscope

• Zeiss Axio Observer Z1
• Motorized XY scanning stage
• Motorized focus control (Z) with Definite Focus
• Stage Top Chamber with Environmental Support (temperature, humidity and CO2)

Objectives

• 5x, 0.13 NA
• 20x, 0.8 NA
• 40x, 1.2 NA, Water Corr
• 63x, 1.3 NA

Illumination

• Transmitted Light Illuminator (Halogen lamp)
• Epi Fluorescence Illuminator (HXP 120V; widefield visual observation)
• Laser: 405nm, multiline argon (458nm, 488nm, 514nm), 561nm, 633nm

Detectors

• 2 Conventional PMTs
• 1 High sensitivity GaAsP detector
• 1 AiryScan Super Resolution Detector

Capabilities

• Microbeam laser unit (pulsed 355nm) allowing laser cutting and non-contact capture of both fixed and live samples
• Multichannel imaging
• Live cell imaging
• Timelapse, time series, timed capture

Microscope

• Zeiss Axio Observer Z1
• Motorized XY scanning stage and focus control (Z)
• Stage top chamber with environmental support (temperature, humidity and CO2)

Objectives

• Fluar 5x, 0.25 NA
• Fluar 10x, 0.25 NA
• LD Plan-Neofluar 20x, 0.4 NA Corr
• LD Plan-Neofluar 40x, 0.6 NA Corr

Illumination

• Transmitted light illuminator (Halogen lamp)
• Epi fluorescence illuminator (HXP 120V)
• Filter based excitation/emission for DAPI, GFP, mRFP, Cy5

Cameras

• AxioCam ICc 5 - color camera
• AxioCam MRm - monochrome camera

Capabilities

• Two-photon excitation with dual channel detection allows for deep tissue penetration and imaging of GFP together with mCherry/TdTomato
• Multiarea (XYZ), time-lapse imaging with environment control (solution perfusion, O2/CO2, temperature) for live imaging in tissue sections.
• Photostimulation, uncaging

Microscope

• Olympus BX61WI upright microscope with motorized focus control (Z drive)
• Fully motorized stage for precise repeatable movement (XY) of the specimen with respect to the fixed microscope
• Stage top chamber with environmental support (temperature, humidity and C2)

Objectives

• 10x, 0.3 NA water immersion
• 40x, 0.8 NA water immersion

Illumination

• Transmitted light illuminator (Halogen lamp)
• Ti:S laser tuneable between 690 to 1040 nm

Detectors

• 2 Photomultiplier detector pairs (non-descanned, above stage and substage): for high sensitivity capture of emitted fluorescence (both reflected and transmitted). Equipped with emission filters for two channel acquisition of GFP and mCherry/TdTomato
• Transmitted light detector with Dodt gradient contrast

Capabilities

• Two-photon excitation with dual channel detection allows deep tissue penetration and fluorescence imaging
• The system is adaptable, with optional stage and specimen chamber available for imaging from tissue sections, but is primarily designed for imaging from live rodents, for example via a cranial window (thinned skull or open-skull glass window) at depths of up to 1 mm from the brain surface
• Capable of live imaging at video frame paired with simultaneous photostimulation

Microscope

• Olympus BX61WI upright microscope with motorized focus control (Z drive)
• Fully motorized stage for precise repeatable movement (XY) of the specimen with respect to the fixed microscope is available
• Stage top chamber with environmental support (temperature, humidity and O2/CO2) is available for use

Objectives

• 10x, 0.3 NA water immersion
• 20x, 1.0 NA water immersion
• 40x, 0.8 NA water immersion

Illumination

• Transmitted light illuminator (Halogen lamp)
• Fast resonance scanner with inputs for two Ti:S lasers tuneable between 690 to 1040 nm, one of which is dedicated for photostimulation

Detectors

• Two high sensitivity GaAsP detectors, configured to capture emitted fluorescence from up to three distinct fluorophores (e.g. blue, green or red fluorescent emitters) in real-time
• Transmitted light detector with Dodt gradient contrast